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p5e elavl3  (Addgene inc)


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    Structured Review

    Addgene inc p5e elavl3
    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    P5e Elavl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p5e elavl3/product/Addgene inc
    Average 92 stars, based on 3 article reviews
    p5e elavl3 - by Bioz Stars, 2026-06
    92/100 stars

    Images

    1) Product Images from "Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity"

    Article Title: Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity

    Journal: bioRxiv

    doi: 10.64898/2026.02.20.707049

    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
    Figure Legend Snippet: Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Techniques Used: Over Expression, Clone Assay, Expressing, Transgenic Assay, Quantitative RT-PCR, Imaging



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    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( <t>elavl3::c3</t> ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.
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    Image Search Results


    Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Journal: bioRxiv

    Article Title: Pre-clinical models of idiopathic scoliosis implicate sex-specific roles for complement activity in modulating spinal curve severity

    doi: 10.64898/2026.02.20.707049

    Figure Lengend Snippet: Pan-neuronal overexpression of c3 in the sspo ( dmh4/+ ) IS model leads to a female-specific increase in spinal curve severity. (A) A Tg( elavl3::c3 ) transgene driving pan-neuronal overexpression of c3 was cloned, and (B) expression levels in stable transgenic animals validated by qRT-PCR. (C) Tg( elavl3::c3 ) expression does not cause axial phenotypes in a wildtype background. (D) Quantification of scoliosis penetrance in sspo ( dmh4/+ ) and sspo ( dmh4/+ ); Tg( elavl3::c3 ) siblings revealed no significant difference between cohorts (Two-sided Fisher’s exact test, p=0.6346; if separated by sex: females, p>0.9999; males, p>0.9999). To quantify the effect of c3 overexpression on sspo ( dmh4/+ ) scoliosis severity in male (E) and female (I) fish, uCT imaging was performed, followed by Cobb angle analysis (F-H, J-L). The scoliosis phenotype was not penetrant in 4 fish (Tg-male, n=1; Tg+ male, n=2; Tg+ female, n=1). These data points have been eliminated from the analysis of scoliosis severity. Scale bars = 5mm.

    Article Snippet: 2) zebrafish, p5E-elavl3 (Addgene #75025), pME-c3b.2, and p3E-polyA were recombined into pDEST Tol2 HR2 transgenesis vector.

    Techniques: Over Expression, Clone Assay, Expressing, Transgenic Assay, Quantitative RT-PCR, Imaging